By Gerhard Flachowsky
This publication includes 15 chapters that debate using transgenic vegetation as animal feeds. issues contain the constraints of transgenic vegetation for animal food; basics of plant biotechnology; dietary, safeguard and compositional overview of feeds from transgenic vegetation; feeding experiences utilizing transgenic crops; the destiny of transgenic DNA and expressed proteins; the impact of feeds from transgenic vegetation at the composition and caliber of animal items; feed ingredients from transgenic microorganisms; socioeconomics and public recognition of transgenic vegetation. on hand In Print
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Extra info for Animal nutrition with transgenic plants
However, it remains easier not to have to use a selection marker. In experimental conditions where the transformation frequency is sufficiently high, screening for uptake of DNA in the plant cells is feasible by using PCR. In this function, shoots need to be regenerated, allowing part of each individual plant to be sampled in order to extract genomic DNA for PCR analysis. Plants for which a positive signal is obtained in the PCR reaction have integrated the gene of interest. However, this does not guarantee expression of the trait.
Therefore, a method is needed either to kill the non-transformed cells, such that only the transformed growing cells survive, or a visible marker is needed to distinguish the transformed from the non-transformed cells. Selectable marker genes result in a selective advantage for the transformed cells: either (i) because of the expression of an enzyme that inactivates the selective agent (detoxification); or (ii) because of the expression of a resistant variant of the endogenous enzyme that is the target of the selection agent (tolerance).
Delivery of DNA to the plant genome without influencing cell viability negatively. 2. Selection of the transformants, the selectable marker gene and promoter. 3. Regeneration of intact plants. 4. Transmission of the transgenes into the next generations in fertile plants or stable maintenance and expression in vegetatively propagated crops. In summary, transformation aims to create heritable changes in the plant as the result of the uptake and the stable integration of introduced DNA in one of the plant chromosomes.
Animal nutrition with transgenic plants by Gerhard Flachowsky