By R. E. Spier
This ebook provides a state of the art document at the box of animal telephone expertise, a resource of reference for all these desirous about the creation and use of animal telephone items
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Genetic enhancement of protein productivity of animal cells by oncogenes. J. et. al. Antisense-/os RNA Causes Partial Reversion of the Transformed Phenotypes Induced by the c-Ha-ras oncogene, Mol. Cell. 4, 1990. M. Wurm, A. Johnson, Y. Lie, T. Etcheverry and K. P. , South San Francisco, CA 94080,* present adress: ISIS Pharmaceuticals, 2280 Faraday Av. Carlsbad, CA92008 ABSTRACT CHO cells, like many other rodent cells, contain hundreds and perhaps thousands of copies of sequences reminiscent of A-type and C-type retrovirus genomes 1 « 2 · 3 ' 4 .
RESULTS Appearance of highly IL-6 productive clones by transfection of oncogenetransforroed SFME cells with the IL-6 gene. SFME cells are a normal cell line derived from mouse embryo cells estab lished by serum-free culture. Since SFME cells exhibit no crisis and no gross chromosomal aberrations detectable, it is suggested that so many cellular oncogenes will not be activated. SFME cells were transfected with pRc/CMV plasmids expressing oncogenes. Three clones of transformed SFME cells with each oncogene were cotransfected again with the IL-6 ex pressing plasmid and pSV2gpt and transformants were selected in a 96well microplate by MPA resistances.
A. F. Induction of hair growth by implantation of cultured dermal papilla cells. Nature, 1984, 311, 560-562. , Grodzicker, T. and Jaramillo, M. A retrovirus expressing the 12s adenoviral E1A gene product can immortalize epithelial cells from a broad range of rat tissues. Mol. Cell. , 1988, 8, 1036-1044. J. A new technique for the visualization of the cytoskeleton in cultured fibroblasts with Coomassie blue R250. Cell Biol. Int. Rep. 1980, 4, 149-153. 28 IMMORTALISATION OF FLATFISH (PARALICHTHYS OLIVACEUS) LEUKOCYTES BY ONCOGENE TRANSFECTION Tadakazu Tamai*, Nobuyuki Sato*, Shoji Kimura*, Sanetaka Shirahata+ and Hiroki Murakami* *Taiyo Central R&D Institute, Taiyo Fishery Co.
Animal Cell Technology. Developments, Processes and Products by R. E. Spier