New PDF release: Advances in Oxytocin Research. Proceedings of a Symposium

By J. H. M. Pinkerton (Eds.)

ISBN-10: 0080110991

ISBN-13: 9780080110998

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Extra resources for Advances in Oxytocin Research. Proceedings of a Symposium Held on 1st May 1964 Under the Auspices of the Blair–Bell Research Society at the Royal College of Obstetricians and Gynaecologists, Sussex Place, Regent's Park, London, England

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The filter paper sheet was subdivided into appropriate bands and each was eluted with Tyrode's solution. These eluates were re-assayed for oxytocin on the lactating guinea-pig. On all chromatograms synthetic oxytocin (Sandoz) was run in parallel with the test PAPER CHROMATOGRAPHY Butanol-Acetic Acid—Water 1-0- -10 •9- -•9 •8" •8 Θ © -·7 -·6 -•5Rf 5HT ! Θ -·4 -·3 ',ΝοτΑά', •2 -·1 Origin- -Origin FIG. 2. Composite diagram representing the movement of various substances in the sheet paper Chromatographie procedure described in the text: Ox— oxytocin (synthetic); AVT—arginine vasotocin (synthetic); AVP—arginine vasopressin (synthetic); LVP—lysine vasopressin (synthetic); BK—bradykinin (synthetic); P—substance P (highly purified); 5HT—5 hydroxy tryptamine; Nor.

In two cases no significant release of oxytocin was detected while in the third a level of 154/xU/ml plasma was found. As yet our studies on blood taken immediately following parturition are rather limited, but the data so far obtained indicate that the blood oxytocin levels fall rapidly after birth. These studies on the release of oxytocin in the parturient goat are being continued. 5. 30 9 10 Blood sample, no. FIG. 2. Milk-ejection activity (oxytocin) in jugular blood of a goat during parturition.

P. FIG. 3. Intra-mammary pressure responses in the guinea-pig, to close intraarterial injection of synthetic oxytocin are shown as pressure recordings (below) and are plotted as a log. dose-response curve (above). The relationship is satisfactorily linear for assay purposes. All doses are in microunits. In the interpretation of our results, we place great emphasis on the specificity of this bioassay preparation which eliminates from consideration the presence in our extracts of many contaminants which could show high activity if the rat uterine assay were used.

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Advances in Oxytocin Research. Proceedings of a Symposium Held on 1st May 1964 Under the Auspices of the Blair–Bell Research Society at the Royal College of Obstetricians and Gynaecologists, Sussex Place, Regent's Park, London, England by J. H. M. Pinkerton (Eds.)


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